Three original characters designed by Akira Toriyama W called Kane, Galena, and Prometheus are antagonists of story mode. Eleven additional characters are planned to be added via downloadable content for a total of 51 characters. The game's launch roster features 40 playable characters who come from 16 different series. The match ends when one team depletes the other's health bar. Each stage can also transition to a different location during battle if a character has 50% or more awakening gauge and heavy smashes the other player twice. Awakening skills can be performed when the player's awakening gauge is at 50% or more, and the gauge only builds up when the player takes damage. Characters have three special skills and an awakening skill which functions as an ultimate attack. Similar to J-Stars Victory Vs, these fighters can move and fight in all directions within a 3D arena. Players control one character at a time while the others are used as support, with players able to switch between them during battle while sharing a single health bar. Jump Force is a 3-on-3 tag team arena fighting game. However, a suspicious figure is attempting to gain the Onyx Book and conquer all worlds. In order to fight back, many heroes are recruited to join the "Jump Force" under the leadership of Director Glover and his A.I.
When the real world collides with many of the Shōnen Jump universes, humanity is invaded by the "Venoms", an army of mind-controlled villains lead by Kane and Galena.